Mouse Offspring after Microinjection of Heated Spermatozoa
نویسندگان
چکیده
منابع مشابه
Pregnancies after microinjection of human spermatozoa into human oocytes.
The use of In-Vitro Fertilisation (IVF) has become an accepted treatment for couples with male factor infertility!. However, there must be adequate number of motile spermatozoa it there is to be a favourable outcome. The introduction of Intra Cytoplasmic Sperm Injection (ICS!) technique by Palermo et aP has encouraged an increase in the clinical use of the procedure in men with very poor semen ...
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The objective of this study was to evaluate the spermatozoa quality of the F4 F5 generation of transgenic rabbit males carrying the mWAP-hFVIII gene in comparison to non-transgenic individuals and previous transgenic generations. Semen samples from 4 transgenic rabbits and 10 randomly selected non-transgenic males of the same breed and age were collected once a week (5 ejaculates per male were ...
متن کاملHeated spermatozoa: effects on embryonic development and epigenetics.
BACKGROUND Sperm chromatin is highly condensed and relatively resistant to chemical and physical treatments. The purpose of this study was to explore the highest temperature that sperm can tolerate and still produce live offspring. METHODS Mouse sperm were heated in a water bath at 50, 65, 80 or 95°C for 30 min before they were microinjected into mouse oocytes. Fertilization, embryo developme...
متن کاملHealthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months.
If humans ever start to live permanently in space, assisted reproductive technology using preserved spermatozoa will be important for producing offspring; however, radiation on the International Space Station (ISS) is more than 100 times stronger than that on Earth, and irradiation causes DNA damage in cells and gametes. Here we examined the effect of space radiation on freeze-dried mouse sperm...
متن کاملMouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring.
Genomic imprinting occurs in both male and female gametes during gametogenesis, but the exact time when imprinting begins and ends is unknown. In the present study we injected nuclei of testicular spermatozoa and round spermatids into mature mouse oocytes to see whether these nuclei are able to participate in syngamy and normal embryonic development. If the injected oocytes develop into normal ...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 2001
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod65.5.1518